Intracellular analysis by SERRS and nanoparticles

Stevenson, R. and Senior, L. and Alexander, J . and Geboers, M. and Faulds, K. and Graham, D. (2010) Intracellular analysis by SERRS and nanoparticles. In: Nanotechnology 2010. Nano Science and Technology Institute, Austin, TX, pp. 153-156. ISBN 9781439834152

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Abstract

We report the first use of ‘bare’ nanoparticles as both a cellular delivery vehicle and a spectroscopic detection platform to enable surface enhanced resonance Raman scattering (SERRS) detection. We have shown how this approach can be used to observe the conversion of a specific Raman active substrate whose enzyme is naturally occurring within that cell line. The technique has been used to pinpoint unique Raman signals at specific sites within a cell at nm resolution, suggesting a novel approach for enzyme localization and representing a major advancement upon both current SERRS and fluorescence cell based research. Further we show how addition of an enzyme inhibitor can be used to reduce the SERRS output in a dose dependant manner, a significant breakthrough upon current enzyme cellular analysis.