Intracellular analysis by SERRS and nanoparticles

Stevenson, R. and Senior, L. and Alexander, J . and Geboers, M. and Faulds, K. and Graham, D.; (2010) Intracellular analysis by SERRS and nanoparticles. In: Nanotechnology 2010. Nano Science and Technology Institute, USA, pp. 153-156. ISBN 9781439834152 (http://www.nsti.org/procs/Nanotech2010v3/1/W50.126)

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Abstract

We report the first use of ‘bare’ nanoparticles as both a cellular delivery vehicle and a spectroscopic detection platform to enable surface enhanced resonance Raman scattering (SERRS) detection. We have shown how this approach can be used to observe the conversion of a specific Raman active substrate whose enzyme is naturally occurring within that cell line. The technique has been used to pinpoint unique Raman signals at specific sites within a cell at nm resolution, suggesting a novel approach for enzyme localization and representing a major advancement upon both current SERRS and fluorescence cell based research. Further we show how addition of an enzyme inhibitor can be used to reduce the SERRS output in a dose dependant manner, a significant breakthrough upon current enzyme cellular analysis.

ORCID iDs

Stevenson, R., Senior, L., Alexander, J ., Geboers, M., Faulds, K. ORCID logoORCID: https://orcid.org/0000-0002-5567-7399 and Graham, D. ORCID logoORCID: https://orcid.org/0000-0002-6079-2105;
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