A microfluidics tool for high-throughput, real-time multimodal imaging of nanoparticle-cell interactions

Cunha-Matos, C. A. and Millington, O. M. and Wark, A. W. and Zagnoni, M.; Colin, Stéphane and Morini, Gian Luca and Brandner, Jürgen J., eds. (2014) A microfluidics tool for high-throughput, real-time multimodal imaging of nanoparticle-cell interactions. In: 4th European Conference on Microfluidics, Limerick, 10-12 December 2014. Société hydrotechnique de France, IRL. ISBN 9791093567044

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    The increasing use of nanomaterials for biomedical applications has raised the need for efficient, robust and low-cost high-throughput assessment of nanotoxicity and cell-nanoparticle interactions. Microfluidics provides the tools for high-throughput single-cell functional monitoring, while gold nanorods have unique potential for intracellular tracking and can simultaneously be used as drug carriers. Presented here is a miniaturised platform that integrates these features with a multimodal approach to cell imaging. A microfluidic device allows for trapping of an array of singlecells, followed by the controlled delivery of nanoparticles into the cell array and subsequent real-time multimodal imaging of cellular interactions with functionalised nanoparticles. This system has been successfully used to assess cellnanoparticle interactions at the single-cell level.

    ORCID iDs

    Cunha-Matos, C. A. ORCID logoORCID: https://orcid.org/0000-0001-5528-1725, Millington, O. M. ORCID logoORCID: https://orcid.org/0000-0002-3026-6550, Wark, A. W. ORCID logoORCID: https://orcid.org/0000-0001-8736-7566 and Zagnoni, M. ORCID logoORCID: https://orcid.org/0000-0003-3198-9491; Colin, Stéphane, Morini, Gian Luca and Brandner, Jürgen J.