Rapid quantification and validation of lipid concentrations within liposomes
Roces, Carla B and Kastner, Elisabeth and Stone, Peter and Lowry, Deborah and Perrie, Yvonne (2016) Rapid quantification and validation of lipid concentrations within liposomes. Pharmaceutics, 8 (29). pp. 1-11. ISSN 1999-4923 (https://doi.org/10.3390/pharmaceutics8030029)
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Abstract
Quantification of the lipid content in liposomal adjuvants for subunit vaccine formulation is of extreme importance since this concentration impacts both on efficacy and stability. Within this paper we outline an HPLC-ELSD method that allows for the rapid and simultaneous quantification of lipid concentrations within liposomal systems prepared by three liposomal manufacturing techniques (lipid film hydration, high shear mixing and microfluidics). The ELSD system was used to quantify 4 lipids: 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), cholesterol, dimethyldioctadecylammonium (DDA) and D-(+)-trehalose 6,6’-dibehenate (TDB). The developed method offers rapid, high sensitivity, direct linearity and a good consistency on the responses (R2>0.993 for 4 lipids tested). The corresponding LOD and LOQ were 0.11 and 0.36 mg/mL (DMPC), 0.02 and 0.80 mg/mL (cholesterol), 0.06 and 0.20 mg/mL (DDA), and 0.05 and 0.16 mg/mL (TDB), respectively. HPLC-ELSD was shown to be a rapid and effective method for the quantification of lipids within liposome formulations without the need for lipid extraction processes.
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Item type: Article ID code: 57764 Dates: DateEvent13 September 2016Published2 September 2016AcceptedSubjects: Medicine > Pharmacy and materia medica Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 13 Sep 2016 08:26 Last modified: 11 Nov 2024 11:30 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/57764