Picture of mobile phone running fintech app

Fintech: Open Access research exploring new frontiers in financial technology

Strathprints makes available Open Access scholarly outputs by the Department of Accounting & Finance at Strathclyde. Particular research specialisms include financial risk management and investment strategies.

The Department also hosts the Centre for Financial Regulation and Innovation (CeFRI), demonstrating research expertise in fintech and capital markets. It also aims to provide a strategic link between academia, policy-makers, regulators and other financial industry participants.

Explore all Strathclyde Open Access research...

The EpiTect Methyl qPCR Assay as novel age estimation method in forensic biology

Mawlood, Shakhawan K. and Dennany, Lynn and Watson, Nigel and Pickard, Benjamin S. (2016) The EpiTect Methyl qPCR Assay as novel age estimation method in forensic biology. Forensic Science International, 264. pp. 132-138. ISSN 0379-0738

[img]
Preview
Text (Mawlood-etal-FSI2016-EpiTect-Methyl-qPCR-Assay-as-novel-age-estimation-method-in-forensic-biology)
Mawlood_etal_FSI2016_EpiTect_Methyl_qPCR_Assay_as_novel_age_estimation_method_in_forensic_biology.pdf
Accepted Author Manuscript
License: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 logo

Download (666kB) | Preview

Abstract

Human aging is associated with epigenetic modification of the genome. DNA methylation at cytosines appears currently as the best characterised modification that occurs during the mammalian lifetime. Such methylation changes at regulatory region can provide insights to track contributor age for criminal investigation. The EpiTect Methyl II PCR system (QIAGEN) was used to compare methylation levels of CpG islands in the promoter regions of a number of age related genes, of which four successfully showed changes across the lifespan (NPTX2, KCNQ1DN, GRIA2 and TRIM58). This technique is based on the detection of remaining input genome after digestion with a methylation-sensitive restriction enzyme. This study examined DNA specimens from 80 female subjects of various ages (18-91 years) obtained from blood, using primers designed to flank the studied gene loci. The data obtained from DNA methylation quantification showed successful discrimination among volunteered ages. Overall, the difference between predicted and real age was about 11 years and absolute mean differences (AMD) was only 7.2 years error. We suggest the EpiTect system can be used as fast and simple innovative tool in future forensic age estimation.