SERS active colloidal nanoparticles for the detection of small blood biomarkers using aptamers

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Marks, Haley and Mabbott, Samuel and Jackson, George W. and Graham, Duncan and Cote, Gerard L.; Parak, Wolfgang J. and Osinski, Marek and Liang, Xing-Jie, eds. (2015) SERS active colloidal nanoparticles for the detection of small blood biomarkers using aptamers. In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Proceedings of SPIE . SPIE, USA. ISBN 9781628414288 (https://doi.org/10.1117/12.2078869)

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Abstract

Functionalized colloidal nanoparticles for SERS serve as a promising multifunctional assay component for blood biomarker detection. Proper design of these nanoprobes through conjugation to spectral tags, protective polymers, and sensing ligands can provide experimental control over the sensitivity, range, reproducibility, particle stability, and integration with biorecognition assays. Additionally, the optical properties and degree of electromagnetic SERS signal enhancement can be altered and monitored through tuning the nanoparticle shape, size, material and the colloid's local surface plasmon resonance (LSPR). Aptamers, synthetic affinity ligands derived from nucleic acids, provide a number of advantages for biorecognition of small molecules and toxins with low immunogenicity. DNA aptamers are simpler and more economical to produce at large scale, are capable of greater specificity and affinity than antibodies, are easily tailored to specific functional groups, can be used to tune inter-particle distance and shift the LSPR, and their intrinsic negative charge can be utilized for additional particle stability.<sup>1,2</sup> Herein, a "turn-off" competitive binding assay platform involving two different plasmonic nanoparticles for the detection of the toxin bisphenol A (BPA) using SERS is presented. A derivative of the toxin is immobilized onto a silver coated magnetic nanoparticle (Ag@MNP), and a second solid silver nanoparticle (AgNP) is functionalized with the BPA aptamer and a Raman reporter molecule (RRM). The capture (Ag@MNP) and probe (AgNP) particles are mixed and the aptamer binding interaction draws the nanoparticles closer together, forming an assembly that results in an increased SERS signal intensity. This aptamer mediated assembly of the two nanoparticles results in a 100x enhancement of the SERS signal intensity from the RRM. These pre-bound aptamer/nanoparticle conjugates were then exposed to BPA in free solution and the competitive binding event was monitored by the decrease in SERS intensity.

ORCID iDs

Marks, Haley, Mabbott, Samuel ORCID logoORCID: https://orcid.org/0000-0003-4926-5467, Jackson, George W., Graham, Duncan ORCID logoORCID: https://orcid.org/0000-0002-6079-2105 and Cote, Gerard L.; Parak, Wolfgang J., Osinski, Marek and Liang, Xing-Jie
  • Item type:Book Section
    ID code:57368
    Dates:
    Date
    Event
    12 March 2015
    Published
    12 March 2015
    Accepted
    Notes:Marks, H., Mabbott, S., Jackson, G. W., Graham, D., & Cote, G. L. "SERS active colloidal nanoparticles for the detection of small blood biomarkers using aptamers" Progress in Biomedical Optics and Imaging - Proceedings of SPIE. [93381C], W. J. Parak, M. Osinski, & X-J. Liang (Eds.), Vol. 9338, 93381C (2015). Copyright 2015 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. http://dx.doi.org/10.1117/12.2078869
    Subjects:Science > Physics > Optics. Light
    Medicine > Medicine (General)
    Department:Faculty of Science > Pure and Applied Chemistry
    Depositing user: Pure Administrator
    Date deposited:11 Aug 2016 09:20
    Last modified:21 Nov 2024 01:27
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/57368
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