Transitioning from multi-phase to single-phase microfluidics for long-term culture and treatment of multicellular spheroids

McMillan, Kay S. and Boyd, Marie and Zagnoni, Michele (2016) Transitioning from multi-phase to single-phase microfluidics for long-term culture and treatment of multicellular spheroids. Lab on a Chip, 16 (18). pp. 3548-3557. ISSN 1473-0197

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    Abstract

    When compared to methodologies based on low adhesion or hanging drop plates, droplet microfluidics offers several advantages for the formation and culture of multicellular spheroids, such as the potential for higher throughput screening and the use of reduced cell numbers, whilst providing increased stability for plate handling. However, a drawback of the technology is its characteristic compartmentalisation which limits the nutrients available to cells within an emulsion and poses challenges to the exchange of the encapsulated solution, often resulting in short-term cell culture and/or viability issues. The aim of this study was to develop a multi-purpose microfluidic platform that combines the high-throughput characteristics of multi-phase flows with that of ease of perfusion typical of single-phase microfluidics. We developed a versatile system to upscale the formation and long-term culture of multicellular spheroids for testing anticancer treatments, creating and array of fluidically addressable, compact spheroids that could be cultured in either medium or within a gel scaffold. The work provides proof-of-concept results for using this system to test both chemo- and radio-therapeutic protocols using in vitro 3D cancer models.

    Author(s):McMillan, Kay S. ORCID logoORCID: https://orcid.org/0000-0001-8710-2496, Boyd, Marie ORCID logoORCID: https://orcid.org/0000-0003-4120-2218 and Zagnoni, Michele ORCID logoORCID: https://orcid.org/0000-0003-3198-9491
    Item type:Article
    ID code:57147
    Keywords:droplet microfluidics, spheroids, drug screening assays, antitumour, microfluidic platform, multi-phase flows, anticancer treatments, Pharmacy and materia medica, Technology, Pharmaceutical Science
    Subjects:Medicine > Pharmacy and materia medica
    Technology
    Department:Faculty of Engineering > Electronic and Electrical Engineering
    Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
    Technology and Innovation Centre > Advanced Science and Technology
    Technology and Innovation Centre > Bionanotechnology
    Depositing user: Pure Administrator
    Date deposited:27 Jul 2016 15:46
    Last modified:18 Oct 2019 01:05
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/57147
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