Depth-specific optogenetic control in vivo with a scalable, high density µLED neural probe
Scharf, Robert and Tsunematsu, Tomomi and McAlinden, Niall and Dawson, Martin D. and Sakata, Shuzo and Mathieson, Keith (2016) Depth-specific optogenetic control in vivo with a scalable, high density µLED neural probe. Scientific Reports, 6. 28381. ISSN 2045-2322
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Abstract
Controlling neural circuits is a powerful approach to uncover a causal link between neural activity and behaviour. Optogenetics has been widely adopted by the neuroscience community as it offers cell-type-specific perturbation with millisecond precision. However, these studies require light delivery in complex patterns with cellular-scale resolution, while covering a large volume of tissue at depth in vivo. Here we describe a novel high-density silicon-based microscale light-emitting diode (µLED) array, consisting of up to ninety-six 25 µm-diameter µLEDs emitting at a wavelength of 450 nm with a peak irradiance of 400 mW/mm2. A width of 100 µm, tapering to a 1 µm point, and a 40 µm thickness help minimise tissue damage during insertion. Thermal properties permit a set of optogenetic operating regimes, with ~0.5°C average temperature increase. We demonstrate depth-dependent activation of mouse neocortical neurons in vivo, offering an inexpensive novel tool for the precise manipulation of neural activity.
Creators(s): |
Scharf, Robert, Tsunematsu, Tomomi, McAlinden, Niall ![]() ![]() ![]() ![]() | Item type: | Article |
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ID code: | 56610 |
Keywords: | neural circuits, neural activity, neuroscience, optogenetics , microscale light-emitting diodes, Optics. Light, Neuroscience. Biological psychiatry. Neuropsychiatry, Atomic and Molecular Physics, and Optics, Neuroscience (miscellaneous) |
Subjects: | Science > Physics > Optics. Light Medicine > Internal medicine > Neuroscience. Biological psychiatry. Neuropsychiatry |
Department: | Faculty of Science > Physics Faculty of Science > Physics > Institute of Photonics Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences |
Depositing user: | Pure Administrator |
Date deposited: | 06 Jun 2016 14:43 |
Last modified: | 06 Jan 2021 09:26 |
Related URLs: | |
URI: | https://strathprints.strath.ac.uk/id/eprint/56610 |
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