Depth-specific optogenetic control in vivo with a scalable, high density µLED neural probe

Scharf, Robert and Tsunematsu, Tomomi and McAlinden, Niall and Dawson, Martin D. and Sakata, Shuzo and Mathieson, Keith (2016) Depth-specific optogenetic control in vivo with a scalable, high density µLED neural probe. Scientific Reports, 6. 28381. ISSN 2045-2322

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    Abstract

    Controlling neural circuits is a powerful approach to uncover a causal link between neural activity and behaviour. Optogenetics has been widely adopted by the neuroscience community as it offers cell-type-specific perturbation with millisecond precision. However, these studies require light delivery in complex patterns with cellular-scale resolution, while covering a large volume of tissue at depth in vivo. Here we describe a novel high-density silicon-based microscale light-emitting diode (µLED) array, consisting of up to ninety-six 25 µm-diameter µLEDs emitting at a wavelength of 450 nm with a peak irradiance of 400 mW/mm2. A width of 100 µm, tapering to a 1 µm point, and a 40 µm thickness help minimise tissue damage during insertion. Thermal properties permit a set of optogenetic operating regimes, with ~0.5°C average temperature increase. We demonstrate depth-dependent activation of mouse neocortical neurons in vivo, offering an inexpensive novel tool for the precise manipulation of neural activity.

    Creators(s): Scharf, Robert, Tsunematsu, Tomomi, McAlinden, Niall ORCID logoORCID: https://orcid.org/0000-0002-0369-8788, Dawson, Martin D. ORCID logoORCID: https://orcid.org/0000-0002-6639-2989, Sakata, Shuzo ORCID logoORCID: https://orcid.org/0000-0001-6796-411X and Mathieson, Keith ORCID logoORCID: https://orcid.org/0000-0002-9517-8076;
    Item type:Article
    ID code:56610
    Keywords:neural circuits, neural activity, neuroscience, optogenetics , microscale light-emitting diodes, Optics. Light, Neuroscience. Biological psychiatry. Neuropsychiatry, Atomic and Molecular Physics, and Optics, Neuroscience (miscellaneous)
    Subjects:Science > Physics > Optics. Light
    Medicine > Internal medicine > Neuroscience. Biological psychiatry. Neuropsychiatry
    Department:Faculty of Science > Physics
    Faculty of Science > Physics > Institute of Photonics
    Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
    Depositing user: Pure Administrator
    Date deposited:06 Jun 2016 14:43
    Last modified:06 Jan 2021 09:26
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/56610
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