A review on continuous-flow microfluidic PCR in droplets : advances, challenges and future
Zhang, Yonghao and Jiang, Hui-Rong (2016) A review on continuous-flow microfluidic PCR in droplets : advances, challenges and future. Analytica Chimica Acta, 914. pp. 7-16. ISSN 0003-2670 (https://doi.org/10.1016/j.aca.2016.02.006)
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Abstract
Significant advances have been made in developing microfluidic polymerase chain reaction (PCR) devices in the last two decades. More recently, microfluidic microdroplet technology has been exploited to perform PCR in droplets because of its unique features. For example, it can prevent crossover contamination and PCR inhibition, is suitable for single-cell and single molecule analyses, and has the potential for system integration and automation. This review will therefore focus on recent developments on droplet-based continuous-flow microfluidic PCR, and the major research challenges. This paper will also discuss a new way of on-chip flow control and a rational design simulation tool, which are required to underpin fully integrated and automated droplet-based microfluidic systems. We will conclude with a scientific speculation of future autonomous scientific discoveries enabled by microfluidic microdroplet technologies.
ORCID iDs
Zhang, Yonghao ORCID: https://orcid.org/0000-0002-0683-7050 and Jiang, Hui-Rong;-
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Item type: Article ID code: 55532 Dates: DateEvent31 March 2016Published13 February 2016Published Online4 February 2016AcceptedSubjects: Technology > Mechanical engineering and machinery Department: Faculty of Engineering > Mechanical and Aerospace Engineering
Technology and Innovation Centre > Advanced Engineering and Manufacturing
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical SciencesDepositing user: Pure Administrator Date deposited: 15 Feb 2016 10:19 Last modified: 03 Dec 2024 01:15 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/55532