Real-time fluorescence lifetime actuation for cell sorting using a CMOS SPAD silicon photomultiplier

Mattioli Della Rocca, Francescopaolo and Nedbal, Jakub and Tyndall, David and Krstajic, Nikola and Li, David Day-Uei and Ameer-Beg, Simon M. and Henderson, Robert K. (2016) Real-time fluorescence lifetime actuation for cell sorting using a CMOS SPAD silicon photomultiplier. Optics Letters, 41 (4). pp. 673-676. ISSN 1539-4794 (https://doi.org/10.1364/OL.41.000673)

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Abstract

Time-correlated single photon counting (TCSPC) is a fundamental fluorescence lifetime measurement technique offering high signal to noise ratio (SNR). However, its requirement for complex software algorithms for histogram processing restricts throughput in flow cytometers and prevents on-the-fly sorting of cells. We present a single-point digital Silicon Photomultiplier (SiPM) detector accomplishing real-time fluorescence lifetime-activated actuation targeting cell sorting applications in flow cytometry. The sensor also achieves burst-integrated fluorescence lifetime (BIFL) detection by TCSPC. The SiPM is a single-chip complementary metal oxide semiconductor (CMOS) sensor employing a 3232 single-photon avalanche diode (SPAD) array and 8 pairs of time-interleaved time to digital converters (TI-TDCs) with a 50 ps minimum timing resolution. The sensor’s pile-up resistant embedded centre of mass method (CMM) processor accomplishes lowlatency measurement and thresholding of fluorescence lifetime. A digital control signal is generated with a 16.6 s latency for cell sorter actuation allowing a maximum cell throughput of 60,000 cells per second and an error rate of 0.6%.

ORCID iDs

Mattioli Della Rocca, Francescopaolo, Nedbal, Jakub, Tyndall, David, Krstajic, Nikola, Li, David Day-Uei ORCID logoORCID: https://orcid.org/0000-0002-6401-4263, Ameer-Beg, Simon M. and Henderson, Robert K.;
  • Item type:Article
    ID code:55446
    Dates:
    Date
    Event
    4 February 2016
    Published
    3 January 2016
    Accepted
    Notes:© 2016 [Optical Society of America.]. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.
    Subjects:Medicine > Pharmacy and materia medica
    Department:Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
    Depositing user: Pure Administrator
    Date deposited:03 Feb 2016 15:00
    Last modified:11 Nov 2024 11:17
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/55446
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