Enzymatically activated emulsions stabilised by interfacial nanofibre networks

Moreira, Inês P. and Ramos Sasselli, Ivan and Cannon, Daniel A. and Hughes, Meghan and Lamprou, Dimitrios A. and Tuttle, Tell and Ulijn, Rein V. (2016) Enzymatically activated emulsions stabilised by interfacial nanofibre networks. Soft Matter, 12 (9). pp. 2623-2631. ISSN 1744-6848

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    Abstract

    We report on-demand formation of emulsions stabilised by interfacial nanoscale networks. These are formed through biocatalytic dephosphorylation and self-assembly of Fmoc(9-fluorenylmethoxycarbonyl)-dipeptide amphiphiles in aqueous/organic mixtures. This is achieved by using alkaline phosphatase which transforms surfactant-like phosphorylated precursors into self-assembling aromatic peptide amphiphiles (Fmoc-tyrosine-leucine, Fmoc-YL) that form nanofibrous networks. In biphasic organic/aqueous systems, these networks form preferentially at the interface thus providing a means of emulsion stabilisation. We demonstrate on-demand emulsification by enzyme addition, even after storage of the biphasic mixture for several weeks. Experimental (Fluorescence, FTIR spectroscopy, fluorescence microscopy, electron microscopy, atomic force microscopy) and computational techniques (atomistic molecular dynamics) are used to characterise the interfacial self-assembly process.

    Author(s):Moreira, Inês P. ORCID logoORCID: https://orcid.org/0000-0002-2571-8583, Ramos Sasselli, Ivan ORCID logoORCID: https://orcid.org/0000-0001-6062-2440, Cannon, Daniel A. ORCID logoORCID: https://orcid.org/0000-0002-3497-2559, Hughes, Meghan, Lamprou, Dimitrios A. ORCID logoORCID: https://orcid.org/0000-0002-8740-1661, Tuttle, Tell and Ulijn, Rein V. ORCID logoORCID: https://orcid.org/0000-0001-7974-3779
    Item type:Article
    ID code:55388
    Keywords:emulsions, nanoscale networks, fluorescence microscopy, Chemistry, Chemistry(all), Condensed Matter Physics
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
    Technology and Innovation Centre > Continuous Manufacturing and Crystallisation (CMAC)
    Technology and Innovation Centre > Bionanotechnology
    Depositing user: Pure Administrator
    Date deposited:28 Jan 2016 11:50
    Last modified:20 Sep 2019 03:07
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/55388
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