Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with Fura-2
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McConnell, G. and Riis, E. (2004) Photonic crystal fibre enables short-wavelength two-photon laser scanning fluorescence microscopy with Fura-2. Physics in Medicine and Biology, 49 (20). pp. 4757-4763. ISSN 0031-9155 (http://dx.doi.org/10.1088/0031-9155/49/20/007)
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We report on a novel and compact reliable laser source capable of short-wavelength two-photon laser scanning fluorescence microscopy based on soliton self-frequency shift effects in photonic crystal fibre. We demonstrate the function of the system by performing two-photon microscopy of smooth muscle cells and cardiac myocytes from the rat pulmonary vein and Chinese hamster ovary cells loaded with the fluorescent calcium indicator fura-2/AM.
ORCID iDs
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Item type: Article ID code: 50 Dates: DateEvent2004PublishedSubjects: Science > Physics > Optics. Light Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Faculty of Science > PhysicsDepositing user: Ms FM Breslin Date deposited: 12 Dec 2005 Last modified: 29 Jan 2025 19:07 URI: https://strathprints.strath.ac.uk/id/eprint/50
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