Frequency-dependent ultrasound-induced transformation in E. coli
Deeks, Jeremy and Windmill, James and Agbeze-Onuma, Maduka and Kalin, Robert M and Argondizza, Peter and Knapp, Charles W (2014) Frequency-dependent ultrasound-induced transformation in E. coli. Biotechnology Letters, 36 (12). pp. 2461-2465. ISSN 0141-5492
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Abstract
Ultrasound-enhanced gene transfer (UEGT) is continuing to gain interest across many disciplines; however, very few studies investigate UEGT efficiency across a range of frequencies. Using a variable frequency generator, UEGT was tested in E. coli at six ultrasonic frequencies. Results indicate frequency can significantly influence UEGT efficiency positively and negatively. A frequency of 61 kHz improved UEGT efficiency by ~70 % higher, but 99 kHz impeded UEGT to an extent worse than no ultrasound exposure. The other four frequencies (26, 133, 174, and 190 kHz) enhanced transformation compared to no ultrasound, but efficiencies did not vary. The influence of frequency on UEGT efficiency was observed across a range of operating frequencies. It is plausible that frequency-dependent dynamics of mechanical and chemical energies released during cavitational-bubble collapse (CBC) are responsible for observed UEGT efficiencies.
Creators(s): |
Deeks, Jeremy, Windmill, James ![]() ![]() | Item type: | Article |
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ID code: | 48944 |
Keywords: | bacteria, plasmid, sonoporation, transformation, ultrasonic, frequency-dependent, ultrasound-induced, e. coli, Electrical engineering. Electronics Nuclear engineering, Engineering (General). Civil engineering (General), Microbiology, Electrical and Electronic Engineering, Civil and Structural Engineering, Applied Microbiology and Biotechnology |
Subjects: | Technology > Electrical engineering. Electronics Nuclear engineering Technology > Engineering (General). Civil engineering (General) Science > Microbiology |
Department: | Faculty of Engineering > Civil and Environmental Engineering Faculty of Engineering > Electronic and Electrical Engineering Technology and Innovation Centre > Sensors and Asset Management |
Depositing user: | Pure Administrator |
Date deposited: | 11 Jul 2014 14:44 |
Last modified: | 20 Jan 2021 21:22 |
Related URLs: | |
URI: | https://strathprints.strath.ac.uk/id/eprint/48944 |
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