Inactivation of mycobacterium paratuberculosis by pulsed electric fields

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Rowan, N.J. and MacGregor, S.J. and Anderson, J.G. and Cameron, Douglas and Farish, O. (2001) Inactivation of mycobacterium paratuberculosis by pulsed electric fields. Applied and Environmental Microbiology, 67 (6). pp. 2833-2836. ISSN 0099-2240 (http://dx.doi.org/10.1128/AEM.67.6.2833-2836.2001)

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Abstract

The influence of treatment temperature and pulsed electric fields (PEF) on the viability of Mycobacterium paratuberculosis cells suspended in 0.1% (wt/vol) peptone water and in sterilized cow's milk was assessed by direct viable counts and by transmission electron microscopy (TEM). PEF treatment at 50°C (2,500 pulses at 30 kV/cm) reduced the level of viable M. paratuberculosis cells by approximately 5.3 and 5.9 log10 CFU/ml in 0.1% peptone water and in cow's milk, respectively, while PEF treatment of M. paratuberculosis at lower temperatures resulted in less lethality. Heating alone at 50°C for 25 min or at 72°C for 25 s (extended high-temperature, short-time pasteurization) resulted in reductions of M. paratuberculosis of approximately 0.01 and 2.4 log10 CFU/ml, respectively. TEM studies revealed that exposure to PEF treatment resulted in substantial damage at the cellular level to M. paratuberculosis.

ORCID iDs

Rowan, N.J., MacGregor, S.J. ORCID logoORCID: https://orcid.org/0000-0002-0808-585X, Anderson, J.G. ORCID logoORCID: https://orcid.org/0000-0003-4151-1619, Cameron, Douglas and Farish, O.;
CORE (COnnecting REpositories)