Enzymatically amplified SPR imaging detection of DNA by exonuclease III digestion of DNA microarrays

Lee, H.J. and Li, Y. and Wark, Alastair and Corn, R.M. (2005) Enzymatically amplified SPR imaging detection of DNA by exonuclease III digestion of DNA microarrays. Analytical Chemistry, 77 (16). pp. 5096-5100. ISSN 0003-2700

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Official URL: https://doi.org/10.1021/ac050815w

Abstract

This paper describes a novel approach utilizing the enzyme exonuclease III in conjunction with 3‘-terminated DNA microarrays for the amplified detection of single-stranded DNA (ssDNA) with surface plasmon resonance (SPR) imaging. When ExoIII and target DNA are simultaneously introduced to a 3‘-terminated ssDNA microarray, hybridization adsorption of the target ssDNA leads to the direction-dependent ExoIII hydrolysis of probe ssDNA strands and the release of the intact target ssDNA back into the solution. Readsorption of the target ssDNA to another probe creates a repeated hydrolysis process that results over time in a significant negative change in SPR imaging signal. Experiments are presented that demonstrate the direction-dependent surface enzyme reaction of ExoIII with double-stranded DNA as well as this new enzymatically amplified SPR imaging process with a 16-mer target ssDNA detection limit of 10−100 pM. This is a 102−103 improvement on previously reported measurements of SPR imaging detection of ssDNA based solely on hybridization adsorption without enzymatic amplification

Author(s):	Lee, H.J., Li, Y., Wark, Alastair ORCID: https://orcid.org/0000-0001-8736-7566 and Corn, R.M.
Item type:	Article
ID code:	44602
Keywords:	exonuclease, enzymatically amplified, SPR imaging detection, DNA, digestion, dna microarrays, Chemistry, Chemistry(all), Biochemistry
Subjects:	Science > Chemistry
Department:	Faculty of Science > Pure and Applied Chemistry Technology and Innovation Centre > Bionanotechnology
Depositing user:	Pure Administrator
Date deposited:	27 Aug 2013 14:35
Last modified:	20 Sep 2019 02:49
URI:	https://strathprints.strath.ac.uk/id/eprint/44602
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