Utilizing ultrathin DNA/poly-lysine multilayer films to create liquid/liquid interfaces : spectroscopic characterization, interfacial reactions and nanoparticle adsorption

Lee, Hye Jin and Wark, A.W. and Corn, R.M. (2007) Utilizing ultrathin DNA/poly-lysine multilayer films to create liquid/liquid interfaces : spectroscopic characterization, interfacial reactions and nanoparticle adsorption. Journal of Physics: Condensed Matter, 19. 375107. ISSN 0953-8984

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Abstract

Alternating electrostatic multilayer adsorption of poly-L-lysine (pLys) and DNA is used to create well-defined biopolymer multilayers for use as an ultrathin aqueous phase in liquid–liquid interfacial measurements. The molecular structure and thickness of the polyelectrolyte multilayers are determined using a combination of polarization modulation FT-IR reflection-absorption spectroscopy (PM-FTIRRAS) and FT-surface plasmon resonance (FT-SPR) thickness measurements. Electroactive species such as ferri/ferrocyanide ions can be incorporated into the DNA/pLys polyelectrolyte multilayers. The ion transport activity of these electroactive films when in contact with 1,2-dichoroethane is verified by electrochemical measurements. Micron-sized patterns of these multilayers are created by either photopatterning, vapour-deposited spot patterning or microfluidic stencil processing, and are used in conjunction with fluorescence and surface plasmon resonance imaging (SPRI) to monitor (i) the intercalation of dye molecules into DNA/pLys ultrathin films, (ii) the electrostatic adsorption of gold nanoparticles onto DNA/pLys multilayers and (iii) the spatially controlled incorporation and reaction of enzymes into patterned biopolymer multilayers.