Improving the understanding of oligonucleotide–nanoparticle conjugates using DNA-binding fluorophores

Guerrini, Luca and Barrett, Lee and Dougan, Jennifer A. and Faulds, Karen and Graham, Duncan (2013) Improving the understanding of oligonucleotide–nanoparticle conjugates using DNA-binding fluorophores. Nanoscale, 5 (10). pp. 4166-4170. ISSN 2040-3372 (https://doi.org/10.1039/c3nr01197f)

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Abstract

A novel fluorescent-based method for characterisation of oligonucleotide-nanoparticle conjugates (ONPCs) is reported. We exploit the ability of the double-stranded DNA-binding dye, SYBR Green I, to develop a powerful analytical tool to investigate the melting properties and hybridisation behavior of ONPCs as well as their corresponding DNA-mediated assemblies.

ORCID iDs

Guerrini, Luca, Barrett, Lee ORCID logoORCID: https://orcid.org/0000-0002-5289-657X, Dougan, Jennifer A., Faulds, Karen ORCID logoORCID: https://orcid.org/0000-0002-5567-7399 and Graham, Duncan ORCID logoORCID: https://orcid.org/0000-0002-6079-2105;
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