Picture of neon light reading 'Open'

Discover open research at Strathprints as part of International Open Access Week!

23-29 October 2017 is International Open Access Week. The Strathprints institutional repository is a digital archive of Open Access research outputs, all produced by University of Strathclyde researchers.

Explore recent world leading Open Access research content this Open Access Week from across Strathclyde's many research active faculties: Engineering, Science, Humanities, Arts & Social Sciences and Strathclyde Business School.

Explore all Strathclyde Open Access research outputs...

Discovery of bioactive metabolites from the leaves of Vitex pinnata using high-throughput flash chromatography

Kamal, Nurkhalida Binti and Clements, Carol and Gray, Alexander and Edrada-Ebel, Ruangelie (2011) Discovery of bioactive metabolites from the leaves of Vitex pinnata using high-throughput flash chromatography. Planta Medica, 77 (12). p. 1348. ISSN 0032-0943

Full text not available in this repository. Request a copy from the Strathclyde author

Abstract

In our study we report the chemical investigation and various biological activities of secondary metabolites isolated from the leaves of Vitex pinnata L. In Malaysia, leaves of V. pinnata were traditionally used to treat cuts and wounds while the bark decoction is used for stomach ache and post-childbirth medicine [1,2]. High-throughput flash chromatography proved to be fast, robust, efficient and reproducible in the isolation and purification of the biologically active natural products. Ten compounds were isolated and chemical investigation revealed that retusin, kaemferol trimetyl ether, pheophytin a, β-sitosterol, and a new diterpene are the major compounds found in this leaves. The structures of all isolated compounds were determined by using 1D and 2D-NMR and also LC-MS. Antioxidant and antibacterial activities were tested on isolated compounds and fractions. In the qualitative antioxidant TLC assay, new diterpene and pheophytin a bleached the DPPH (0.2% in MeOH) purple colour indicating antioxidant activity. Retusin and β-sitosterol isolated from hexane extract showed to have antibacterial activity against Microbacterium marinum. Other bioassays such as antitrypanosomal and cytotoxicity studies are still under investigation. Consequently, this plant is a promising source for various biological activities