Picture of DNA strand

Pioneering chemical biology & medicinal chemistry through Open Access research...

Strathprints makes available scholarly Open Access content by researchers in the Department of Pure & Applied Chemistry, based within the Faculty of Science.

Research here spans a wide range of topics from analytical chemistry to materials science, and from biological chemistry to theoretical chemistry. The specific work in chemical biology and medicinal chemistry, as an example, encompasses pioneering techniques in synthesis, bioinformatics, nucleic acid chemistry, amino acid chemistry, heterocyclic chemistry, biophysical chemistry and NMR spectroscopy.

Explore the Open Access research of the Department of Pure & Applied Chemistry. Or explore all of Strathclyde's Open Access research...

Discovery of bioactive metabolites from the leaves of Vitex pinnata using high-throughput flash chromatography

Kamal, Nurkhalida Binti and Clements, Carol and Gray, Alexander and Edrada-Ebel, Ruangelie (2011) Discovery of bioactive metabolites from the leaves of Vitex pinnata using high-throughput flash chromatography. Planta Medica, 77 (12). p. 1348. ISSN 0032-0943

Full text not available in this repository.Request a copy from the Strathclyde author

Abstract

In our study we report the chemical investigation and various biological activities of secondary metabolites isolated from the leaves of Vitex pinnata L. In Malaysia, leaves of V. pinnata were traditionally used to treat cuts and wounds while the bark decoction is used for stomach ache and post-childbirth medicine [1,2]. High-throughput flash chromatography proved to be fast, robust, efficient and reproducible in the isolation and purification of the biologically active natural products. Ten compounds were isolated and chemical investigation revealed that retusin, kaemferol trimetyl ether, pheophytin a, β-sitosterol, and a new diterpene are the major compounds found in this leaves. The structures of all isolated compounds were determined by using 1D and 2D-NMR and also LC-MS. Antioxidant and antibacterial activities were tested on isolated compounds and fractions. In the qualitative antioxidant TLC assay, new diterpene and pheophytin a bleached the DPPH (0.2% in MeOH) purple colour indicating antioxidant activity. Retusin and β-sitosterol isolated from hexane extract showed to have antibacterial activity against Microbacterium marinum. Other bioassays such as antitrypanosomal and cytotoxicity studies are still under investigation. Consequently, this plant is a promising source for various biological activities