Picture of DNA strand

Pioneering chemical biology & medicinal chemistry through Open Access research...

Strathprints makes available scholarly Open Access content by researchers in the Department of Pure & Applied Chemistry, based within the Faculty of Science.

Research here spans a wide range of topics from analytical chemistry to materials science, and from biological chemistry to theoretical chemistry. The specific work in chemical biology and medicinal chemistry, as an example, encompasses pioneering techniques in synthesis, bioinformatics, nucleic acid chemistry, amino acid chemistry, heterocyclic chemistry, biophysical chemistry and NMR spectroscopy.

Explore the Open Access research of the Department of Pure & Applied Chemistry. Or explore all of Strathclyde's Open Access research...

Sugar functionalised PEGA surfaces support metabolically active hepatocytes

Ambury, R. F. and Merry, C. L. R. and Ulijn, R. V. (2011) Sugar functionalised PEGA surfaces support metabolically active hepatocytes. Journal of Materials Chemistry, 21. pp. 2901-2908. ISSN 0959-9428

Full text not available in this repository. Request a copy from the Strathclyde author

Abstract

We demonstrate sugar functionalised hydrogel surface coatings which enable the retention of hepatocyte-specific cell function during in vitro culture. The materials were designed to exploit a unique characteristic of hepatocyte biology, with β-galactose moieties displayed to allow cellular adhesion via the specific asialoglycoprotein receptors (ASGP-R) expressed on hepatocyte cell surfaces. Polyethylene glycol acrylamide hydrogel (PEGA) was modified with a galactose containing ligand, lactobionic acid (LA) with D-glucuronic acid (GlcA) used as a non-ASGP-R binding control. FT-IR analysis of the resultant gels revealed that the sugars had been covalently incorporated into the material. Fluorescence labelling was used to quantify the degree of saccharide integration. Cell culture experiments showed that hepatocytes attached preferentially to sugar containing gel coatings, when compared to non-functionalised PEGA controls. It was observed that cells on the LA and GlcA containing surfaces were more metabolically active, compared to controls, and proliferated to a monolayer by day 7 in culture and maintained some characteristics of hepatocyte functionality, such as urea synthesis over the course of 7 days. Despite these observations, further investigations using flow cytometry and RT-PCR, proved that cell attachment was unlikely to involve an ASGP-R mediated mechanism.