Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore

Chalmers, Susan and Caldwell, S.T. and Quin, C and Prime, T.A. and James, A.M. and Cairns, Alan and Murphy, M.P. and McCarron, John and Hartley, R.C. (2012) Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore. Journal of the American Chemical Society, 134 (2). pp. 758-761. (https://doi.org/10.1021/ja2077922)

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Abstract

Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.

ORCID iDs

Chalmers, Susan ORCID logoORCID: https://orcid.org/0000-0002-8073-7576, Caldwell, S.T., Quin, C, Prime, T.A., James, A.M., Cairns, Alan, Murphy, M.P., McCarron, John ORCID logoORCID: https://orcid.org/0000-0002-3302-3984 and Hartley, R.C.;
CORE (COnnecting REpositories)