Ca(2)+-stimulated exocytosis in maize coleoptile cells

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Sutter, J U and Homann, U and Thiel, G (2000) Ca(2)+-stimulated exocytosis in maize coleoptile cells. Plant Cell, 12 (7). pp. 1127-1136. ISSN 1040-4651 (https://doi.org/10.​1105/​tpc.​12.​7.​1127)

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Abstract

Changes in membrane capacitance (C(m)) after photolysis of the caged Ca(2)+ compound dimethoxynitrophenamine were studied in protoplasts from maize coleoptiles. Changes in C(m) values resulting from increased concentrations of free Ca(2)+ in the cytoplasm ([Ca(2)+](cyt)) were interpreted as representing changes in [Ca(2)+](cyt)-sensitive exocytosis and endocytosis. A continuous increase in [Ca(2)+](cyt) resulted in a sigmoidal increase in C(m) values with a half-maximal concentration at approximately 1 microM. The steep increase in C(m) values was followed by a variable slow phase in changing C(m) values. When [Ca(2)+](cyt) increased at a rate of 0.6 micromol L(-)(1) sec(-)(1), the initial steep increase in C(m) values lasted approximately 5 to 10 sec. During this time, protoplasts increased in surface area by approximately 2.5%. The biphasic dynamics of [Ca(2)+](cyt)-stimulated increases in C(m) values can be described by a kinetic model containing two pools of vesicles with two [Ca(2)+](cyt)-sensitive steps in the exocytotic pathway.

ORCID iDs

Sutter, J U ORCID logoORCID: https://orcid.org/0000-0002-3782-6338, Homann, U and Thiel, G;
  • Item type:Article
    ID code:39690
    Dates:
    Date
    Event
    July 2000
    Published
    Subjects:Agriculture > Plant culture
    Department:Faculty of Science > Physics
    Depositing user: Pure Administrator
    Date deposited:12 May 2012 10:48
    Last modified:11 Nov 2024 10:08
    URI:https://strathprints.strath.ac.uk/id/eprint/39690
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