Spectroscopic analysis of protein Fe-NO complexes.
Bellota-Anton, Cesar and Munnoch, John and Robb, Kirsty and Adamczyk, Katrin and Candelaresi, Marco and Parker, Anthony W. and Dixon, Ray and Hutchings, Matthew I. and Hunt, Neil and Tucker, Nicholas (2011) Spectroscopic analysis of protein Fe-NO complexes. Biochemical Society Transactions, 39. 1293 -1298. ISSN 0300-5127 (https://doi.org/10.1042/BST0391293)
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The toxic free radical NO (nitric oxide) has diverse biological roles in eukaryotes and bacteria, being involved in signalling, vasodilation, blood clotting and immunity, and as an intermediate in microbial denitrification. The predominant biological mechanism of detecting NO is through the formation of iron nitrosyl complexes, although this is a deleterious process for other iron-containing enzymes. We have previously applied techniques such as UV–visible and EPR spectroscopy to the analysis of protein Fe–NO complex formation in order to study how NO controls the activity of the bacterial transcriptional regulators NorR and NsrR. These studies have analysed NO-dependent biological activity both in vitro and in vivo using diverse biochemical, molecular and spectroscopic methods. Recently, we have applied ultrafast 2D-IR (two-dimensional IR) spectroscopy to the analysis of NO–protein interactions using Mb (myoglobin) and Cc (cytochrome c) as model haem proteins. The ultrafast fluctuations of Cc and Mb show marked differences, indicating altered flexibility of the haem pockets. We have extended this analysis to bacterial catalase enzymes that are known to play a role in the nitrosative stress response by detoxifying peroxynitrite. The first 2D-IR analysis of haem nitrosylation and perspectives for the future are discussed.
ORCID iDs
Bellota-Anton, Cesar, Munnoch, John, Robb, Kirsty ORCID: https://orcid.org/0000-0002-7388-9782, Adamczyk, Katrin, Candelaresi, Marco, Parker, Anthony W., Dixon, Ray, Hutchings, Matthew I., Hunt, Neil ORCID: https://orcid.org/0000-0001-7400-5152 and Tucker, Nicholas ORCID: https://orcid.org/0000-0002-6331-3704;-
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Item type: Article ID code: 35461 Dates: DateEvent2011Published21 September 2011Published OnlineSubjects: UNSPECIFIED Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Faculty of Science > Physics
Technology and Innovation Centre > BionanotechnologyDepositing user: Pure Administrator Date deposited: 31 Oct 2011 15:56 Last modified: 11 Nov 2024 09:58 URI: https://strathprints.strath.ac.uk/id/eprint/35461