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Open Access research which pushes advances in bionanotechnology

Strathprints makes available scholarly Open Access content by researchers in the Strathclyde Institute of Pharmacy & Biomedical Sciences (SIPBS) , based within the Faculty of Science.

SIPBS is a major research centre in Scotland focusing on 'new medicines', 'better medicines' and 'better use of medicines'. This includes the exploration of nanoparticles and nanomedicines within the wider research agenda of bionanotechnology, in which the tools of nanotechnology are applied to solve biological problems. At SIPBS multidisciplinary approaches are also pursued to improve bioscience understanding of novel therapeutic targets with the aim of developing therapeutic interventions and the investigation, development and manufacture of drug substances and products.

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Monitoring of particle growth at a low concentration of a poorly water soluble drug using the NanoSight LM20

Gillespie, Cheska and Halling, Peter and Edwards, Darren (2011) Monitoring of particle growth at a low concentration of a poorly water soluble drug using the NanoSight LM20. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 384 (1-3). pp. 233-239. ISSN 0927-7757

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Abstract

The purpose of this work was to investigate the precipitation of a poorly water soluble drug (tolnaftate) from low, mu M concentration solutions. This was to test the applicability of nanoparticle tracking analysis (NTA; the NanoSight instrument), with comparison to results from dynamic light scattering (DLS). Samples containing 30 mu M of tolnaftate, 1% dimethylsulfoxide (DMSO) (by volume) were prepared by mixing a concentrated DMSO stock solution and an aqueous buffer. The samples were then analysed over time either using the NanoSight instrument or by DLS. Obtaining meaningful results from the former required careful attention to instrument settings. From NTA there was initially a fairly narrow size distribution around 200 nm, with concentration of around 4 x 10(8) nanoparticles/mL. Over 3 h, the particles grew, with increasing polydispersity, and skewed distribution up to 800 nm, whilst the concentration fell to around 1 x 10(8) particles/mL. DLS was consistent in showing the size increase, but could not detect the remaining smaller particles and polydispersity. Conclusions: The growth of particles of a poorly water soluble drug was successfully monitored using NTA, which gives additional information not offered by DLS. Nanoparticle precipitation at the concentrations used here is of relevance to high throughput screening in early drug discovery. (C) 2011 Elsevier B.V. All rights reserved.