Cloning and sequencing of an aspergillus niger gene coding for beta-fructufuranosidase

Somiari, R I and Brzeski, H and Tate, R and Bieleck, S and Polak, J (1997) Cloning and sequencing of an aspergillus niger gene coding for beta-fructufuranosidase. Biotechnology Letters, 19 (12). pp. 1243-1247. ISSN 0141-5492

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Abstract

A 1.7 kb DNA fragment encoding beta-D-fructofuranosidase in a strain of Aspergillus niger was amplified by PCR, inserted into a 4.4 kb cloning vector and sequenced. Comparison of this sequence with suc1 (GenBank) showed that this invertase gene had suffered a single base deletion followed by a single base insertion 49bp further down-stream with the result that 16 amino acids are coded by a different reading frame.