Picture of mobile phone running fintech app

Fintech: Open Access research exploring new frontiers in financial technology

Strathprints makes available Open Access scholarly outputs by the Department of Accounting & Finance at Strathclyde. Particular research specialisms include financial risk management and investment strategies.

The Department also hosts the Centre for Financial Regulation and Innovation (CeFRI), demonstrating research expertise in fintech and capital markets. It also aims to provide a strategic link between academia, policy-makers, regulators and other financial industry participants.

Explore all Strathclyde Open Access research...

Isolation and characterization of insulin-stimulated, high-affinity cAMP phosphodiesterases from rat liver

Houslay, M D and Pyne, N J and Cooper, M E (1988) Isolation and characterization of insulin-stimulated, high-affinity cAMP phosphodiesterases from rat liver. Methods in Enzmology, 159. pp. 751-760. ISSN 0076-6879

Full text not available in this repository. Request a copy from the Strathclyde author

Abstract

The ability of glucagon to elevate intracellular cAMP concentrations in hepatocytes is antagonized by insulin.l This effect is achieved through inhibition of adenylate cyclasC and activation of specific cAMP phosphodiesterases. l,3,4 The activation of two specific cAMP phosphodiesterases can readily be assessed using a rapid Percoll gradient fractionation procedure to resolve the various membrane fractions. 1 Both of these enzymes express a high affinity and specificity for cAMP. One of them is a peripheral protein found associated exclusively with the plasma membrane 5 and is activated by its phosphorylation, which is triggered by insulin. 6 The other is associated with an as yet undefined intracellular dense-vesicle fraction.~ The mechanism of its activation has yet to be defined but may involve membrane processing/translocation reactions. 7 The dense-vesicle enzyme can also be activated by glucagon through a process which is distinct from that whereby insulin elicits its activation.