Effect of 405 nm high-intensity narrow-spectrum light on fibroblast populated collagen lattices : an in vitro model of wound healing
Mcdonald, Richard and Grant, M. Helen and Macgregor, Scott J. and Anderson, John G. and Maclean, Michelle (2011) Effect of 405 nm high-intensity narrow-spectrum light on fibroblast populated collagen lattices : an in vitro model of wound healing. Journal of Biomedical Optics, 16 (4). 048003. ISSN 1083-3668 (https://doi.org/10.1117/1.3561903)
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High-intensity narrow-spectrum (HINS) 405-nm light is a novel technology developed to address the significant problem of health-care associated infection. Its potential for wound-decontamination applications is assessed on mammalian cells and bacteria. The fibroblast-populated collagen lattice (FPCL) is used as an in vitro model of wound healing, and the effect of HINS light on contraction is examined. Effects on cell proliferation, morphological changes, and α-smooth muscle actin (α-SMA) expression are investigated. Bactericidal effects are assessed using the bacterium Staphylococcus epidermidis. Low doses of HINS light were found to have no significant inhibitory effects on FPCL contraction, cell proliferation, or α-SMA expression. Doses of up to 18 Jcm−2 had no significant inhibitory effects on FPCL cell numbers, and this dose was shown to cause almost complete inactivation of bacteria. These results show that HINS light has potential for disinfection applications without adversely influencing wound healing.
ORCID iDs
Mcdonald, Richard, Grant, M. Helen ORCID: https://orcid.org/0000-0002-7712-404X, Macgregor, Scott J. ORCID: https://orcid.org/0000-0002-0808-585X, Anderson, John G. ORCID: https://orcid.org/0000-0003-4151-1619 and Maclean, Michelle ORCID: https://orcid.org/0000-0001-5750-0397;-
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Item type: Article ID code: 34729 Dates: DateEventApril 2011Published30 April 2011Published OnlineSubjects: Technology > Engineering (General). Civil engineering (General) > Bioengineering Department: Faculty of Engineering > Bioengineering
Faculty of Engineering > Electronic and Electrical EngineeringDepositing user: Pure Administrator Date deposited: 19 Oct 2011 14:26 Last modified: 23 Nov 2024 20:10 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/34729