Quantitative SERRS for DNA sequence analysis

Graham, Duncan and Faulds, Karen (2008) Quantitative SERRS for DNA sequence analysis. Chemical Society Reviews, 37 (5). pp. 1042-1051. ISSN 0306-0012 (https://doi.org/10.1039/b707941a)

Abstract

SERRS is an extremely sensitive and selective technique which when applied to the detection of labelled DNA sequences allows detection limits to be obtained which rival, and in most cases are better than, fluorescence. In this tutorial review the conditions are explored which enable the successful detection of DNA using SERRS. The enhancing surface which is used is crucial and in this case suspensions of nanoparticles were the focus as they allow quantitative behaviour to be achieved in systems analogous to current fluorescence based approaches. The aggregation conditions required to obtain SERRS of DNA affect the sensitivity and the reproducibility and we describe the use of spermine as an effective aggregating agent to achieve excellent reproducibility and sensitivity. The nature of the label which is used, be it fluorescent or non fluorescent, positively or negatively charged, also affects the SERRS response and these conditions are again discussed. Finally, we show how to detect a specific target DNA sequence in a meaningful diagnostic assay using SERRS and how the approaches described previously in the review are vital to the success of such approaches.

ORCID iDs

Graham, Duncan ORCID: https://orcid.org/0000-0002-6079-2105

Faulds, Karen ORCID: https://orcid.org/0000-0002-5567-7399

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• Item metadata

  Item type:	Article
  ID code:	33679
  Dates:	Date Event 2008 Published 31 March 2008 Published Online
  Subjects:	UNSPECIFIED
  Department:	Faculty of Science > Pure and Applied Chemistry
  Depositing user:	Pure Administrator
  Date deposited:	20 Oct 2011 15:05
  Last modified:	11 Nov 2024 09:51
  URI:	https://strathprints.strath.ac.uk/id/eprint/33679