DNA detection using enzymatic signal production and SERS

Dougan, Jennifer A. and MacRae, Douglas and Graham, Duncan and Faulds, Karen (2011) DNA detection using enzymatic signal production and SERS. Chemical Communications (London), 47 (16). pp. 4649-4651. ISSN 0009-241X

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Official URL: https://doi.org/10.1039/c0cc05216g

Abstract

A novel DNA assay is demonstrated which relies on the production of a surface enhanced resonance Raman scattering (SERS) signal following sequence specific hybridisation and enzyme hydrolysis. This method achieves high sensitivity and specificity that can be applied to the detection of unlabelled duplex DNA and is the first step towards a SERS based signal amplification approach.

Creators(s):	Dougan, Jennifer A., MacRae, Douglas, Graham, Duncan ORCID: https://orcid.org/0000-0002-6079-2105 and Faulds, Karen ORCID: https://orcid.org/0000-0002-5567-7399;
Item type:	Article
ID code:	33652
Keywords:	resonance ram-scattering, exonuclease, nanoparticles, fluorescence, lambda, TIC - Bionanotechnology, Physical and theoretical chemistry, Chemistry(all)
Subjects:	Science > Chemistry > Physical and theoretical chemistry
Department:	Faculty of Science > Pure and Applied Chemistry Technology and Innovation Centre > Bionanotechnology
Depositing user:	Pure Administrator
Date deposited:	17 Oct 2011 13:42
Last modified:	20 Jan 2021 19:34
URI:	https://strathprints.strath.ac.uk/id/eprint/33652
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