Effect of solid-state buffers on the catalytic activity of papain in low water media

Theppakorn, T. and Kanasawud, P. and Halling, P.J. (2003) Effect of solid-state buffers on the catalytic activity of papain in low water media. Enzyme and Microbial Technology, 32 (7). pp. 828-836. ISSN 0141-0229 (http://dx.doi.org/10.1016/S0141-0229(03)00058-9)

Abstract

The catalytic activity of papain in the synthesis of Z-Gly-Phe- NH2 in tert-butanol has been studied in the presence of solid- state acid-base buffers (acids and their sodium salts). All buffer pairs tested reduced the reaction rate compared with the control, particularly the most acidic and basic (assessed by either aqueous pK(a) or the response of an organic phase indicator). The highest rates, close to the control, were found with glutamic acid/glutamate-Na, PIPES/PIPES-Na and NaH2PO4/Na2HPO4. However, these pairs were unable to erase the pH memory phenomenon, or to overcome the effect of spiking with acetic acid. Hence, at least these buffers do not seem to be able to affect the protonation state and catalytic activity of papain. In the last aqueous solution before drying, the presence of activating agents (cysteine plus EDTA) was more important than buffer ions.

ORCID iDs

Halling, P.J. ORCID: https://orcid.org/0000-0001-5077-4088

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• Item metadata

  Item type:	Article
  ID code:	312
  Dates:	Date Event 12 June 2003 Published
  Subjects:	Science > Chemistry
  Department:	Faculty of Science > Pure and Applied Chemistry
  Depositing user:	Users 16 not found.
  Date deposited:	10 Mar 2006
  Last modified:	11 Nov 2024 08:25
  URI:	https://strathprints.strath.ac.uk/id/eprint/312