Affinity chromatography in dynamic combinatorial libraries: one-pot amplification and isolation of a strongly binding receptor

Besenius, P. and Cormack, P.A.G. and Ludlow, R.F. and Otto, S.R. and Sherrington, D.C. (2010) Affinity chromatography in dynamic combinatorial libraries: one-pot amplification and isolation of a strongly binding receptor. Organic and Biomolecular Chemistry, 8 (10). pp. 2414-2418. ISSN 1477-0520

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Abstract

We report the one-pot amplification and isolation of a nanomolar receptor in a multibuilding block aqueous dynamic combinatorial library using a polymer-bound template. By appropriate choice of a poly(N,N-dimethylacrylamide)-based support, unselective ion-exchange type behaviour between the oppositely charged cationic guest and polyanionic hosts was overcome, such that the selective molecular recognition arising in aqueous solution reactions is manifest also in the analogous templated solid phase DCL syntheses. The ability of a polymer bound template to identify and isolate a synthetic receptor via dynamic combinatorial chemistry was not compromised by the large size of the library, consisting of well over 140 theoretical members, demonstrating the practical advantages of a polymer-supported DCL methodology.

Author(s):Besenius, P., Cormack, P.A.G., Ludlow, R.F., Otto, S.R. and Sherrington, D.C.
Item type:Article
ID code:27536
Keywords:acrylamides, amantadines, chromatography, high pressure liquid, nanostructures, Chemistry, Biochemistry, Organic Chemistry, Physical and Theoretical Chemistry
Subjects:Science > Chemistry
Department:Faculty of Science > Pure and Applied Chemistry
Depositing user: Ms Carol Khalaf
Date deposited:30 Sep 2010 15:28
Last modified:03 Jan 2019 12:37
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URI:https://strathprints.strath.ac.uk/id/eprint/27536
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