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Open Access research with a European policy impact...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of Open Access research papers by Strathclyde researchers, including by researchers from the European Policies Research Centre (EPRC).

EPRC is a leading institute in Europe for comparative research on public policy, with a particular focus on regional development policies. Spanning 30 European countries, EPRC research programmes have a strong emphasis on applied research and knowledge exchange, including the provision of policy advice to EU institutions and national and sub-national government authorities throughout Europe.

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Effect of water activity on the rate profile of subtilisin Carlsberg in toluene in the presence of an organo-soluble acid- base buffer

Harper, N. and Dolman, M. and Moore, B.D. and Halling, P.J. (2001) Effect of water activity on the rate profile of subtilisin Carlsberg in toluene in the presence of an organo-soluble acid- base buffer. Enzyme and Microbial Technology, 29 (6-7). pp. 413-416. ISSN 0141-0229

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Abstract

The ionisation state of the enzyme and the water activity (alpha (w)) of the system are two important factors which affect catalytic activity in low-water organic solvents. Here we investigate the interaction between effects of alpha (w) and the ionisation state of subtilisin Carlsberg, controlled by an organo-soluble dendrimer buffer. It was shown that alpha (w) does not have a major effect on the relationship between rate of transesterification in toluene and buffer ratio, [sodium salt]/[acid]. The weak dependence on alpha (w) (between 0.54 and 0.85) probably reflects compensating effects on ionisation equilibria for the buffer and the carboxyl groups on the enzyme. (C) 2001 Elsevier Science Inc. All rights reserved.