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Detection of a DNA sequence by surface enhanced resonance raman scattering of a modified dna probe

Graham, Duncan and Smith, W. Ewen and Linacre, Adrian M. T. and Watson, Nigel D. and White, Peter C. (1998) Detection of a DNA sequence by surface enhanced resonance raman scattering of a modified dna probe. In: Progress in Forensic Genetics 7: Proceedings of the 17th International ISFH Congress. International Congress Series (1167). Elsevier, pp. 6-8. ISBN 0444829652

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Abstract

The technique we are reporting, Surface Enhanced Resonance Raman Scattering, SERRS, exploits the light scattering, or Raman scattering, produced when a light beam illuminates molecules. The effect is weak and depends on the molecular structure so that some molecules are weaker scatters than others are. Accordingly signals from strong scatters can be detected in the presence of weaker ones such as water. The effect is enhanced up to 106 fold if the analyte is adsorbed onto a roughened metal surface, i.e. Surface Enhanced Resonance Scattering (SERS). A further enhancement is achieved by utilizing a dye as the analyte and by tuning the laser excitation frequency to the maximum of the dye chromophore to obtain resonance scattering from the surface i.e. SERRS.