Single and double stranded DNA detection using locked nucleic acid (LNA) functionalized nanoparticles
McKenzie, F. and Stokes, R.J. and Faulds, K. and Graham, D. (2008) Single and double stranded DNA detection using locked nucleic acid (LNA) functionalized nanoparticles. Proceedings of SPIE, 7035. J350-J350. ISSN 0277-786X (https://doi.org/10.1117/12.794965)
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Abstract
Gold and silver nanoparticles functionalized with oligonucleotides can be used for the detection of specific sequences of DNA. We show that gold nanoparticles modified with locked nucleic acid (LNA) form stronger duplexes with a single stranded DNA target and offer better discrimination against single base pair mismatches than analogous DNA probes. Our LNA nanoparticle probes have also been used to detect double stranded DNA through triplex formation, whilst still maintaining selectivity for only complementary targets. Nanoparticle conjugates embedded with suitable surface enhanced resonance Raman scattering (SERRS) labels have been synthesized enabling simultaneous detection and identification of multiple DNA targets.
ORCID iDs
McKenzie, F., Stokes, R.J., Faulds, K. ORCID: https://orcid.org/0000-0002-5567-7399 and Graham, D. ORCID: https://orcid.org/0000-0002-6079-2105;-
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Item type: Article ID code: 18107 Dates: DateEvent29 August 2008PublishedSubjects: Science > Chemistry Department: Faculty of Science > Pure and Applied Chemistry Depositing user: Strathprints Administrator Date deposited: 22 Apr 2010 17:57 Last modified: 02 Sep 2024 01:08 URI: https://strathprints.strath.ac.uk/id/eprint/18107