Single and double stranded DNA detection using locked nucleic acid (LNA) functionalized nanoparticles

McKenzie, F. and Stokes, R.J. and Faulds, K. and Graham, D. (2008) Single and double stranded DNA detection using locked nucleic acid (LNA) functionalized nanoparticles. In: Biosensing, 2008-08-12. (http://dx.doi.org/10.1117/12.794965)

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Abstract

Gold and silver nanoparticles functionalized with oligonucleotides can be used for the detection of specific sequences of DNA. We show that gold nanoparticles modified with locked nucleic acid (LNA) form stronger duplexes with a single stranded DNA target and offer better discrimination against single base pair mismatches than analogous DNA probes. Our LNA nanoparticle probes have also been used to detect double stranded DNA through triplex formation, whilst still maintaining selectivity for only complementary targets. Nanoparticle conjugates embedded with suitable surface enhanced resonance Raman scattering (SERRS) labels have been synthesized enabling simultaneous detection and identification of multiple DNA targets.

ORCID iDs

McKenzie, F., Stokes, R.J., Faulds, K. ORCID logoORCID: https://orcid.org/0000-0002-5567-7399 and Graham, D. ORCID logoORCID: https://orcid.org/0000-0002-6079-2105;
  • Item type:Conference or Workshop Item(Paper)
    ID code:18107
    Dates:
    Date
    Event
    29 August 2008
    Published
    Keywords:gold nanoparticles, silver nanoparticles, DNA, locked nucleic acid, LNA, Chemistry
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Depositing user: Strathprints Administrator
    Date deposited:22 Apr 2010 17:57
    Last modified:20 May 2023 03:17
    URI:https://strathprints.strath.ac.uk/id/eprint/18107
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