Picture of boy being examining by doctor at a tuberculosis sanatorium

Understanding our future through Open Access research about our past...

Strathprints makes available scholarly Open Access content by researchers in the Centre for the Social History of Health & Healthcare (CSHHH), based within the School of Humanities, and considered Scotland's leading centre for the history of health and medicine.

Research at CSHHH explores the modern world since 1800 in locations as diverse as the UK, Asia, Africa, North America, and Europe. Areas of specialism include contraception and sexuality; family health and medical services; occupational health and medicine; disability; the history of psychiatry; conflict and warfare; and, drugs, pharmaceuticals and intoxicants.

Explore the Open Access research of the Centre for the Social History of Health and Healthcare. Or explore all of Strathclyde's Open Access research...

Image: Heart of England NHS Foundation Trust. Wellcome Collection - CC-BY.

Effects of 2 phospholipases a2 from the australian king brown snake (pseudechis-australis) on isolated nerve-muscle preparations

Rowan, E.G. and Harvey, A.L. and Tamiya, N. (1985) Effects of 2 phospholipases a2 from the australian king brown snake (pseudechis-australis) on isolated nerve-muscle preparations. Toxicon, 23 (4). p. 606. ISSN 1879-3150

Full text not available in this repository. Request a copy from the Strathclyde author

Abstract

The venom from P. australis is lethal and has phospholipase A activity. Two phospholipases A (Pa-ll and Pa-13) have been isolated; Pa-ll has an LDs0 value of 0.23 ~g/g (i.v.) in mice, whereas Pa-13 was not lethal at 7.4 ~g/g (Nishlda et aI., 1985). In chick biventer cervlcis, Pa-11 (2-20 ~g/ml) reduced responses to indirect stimulation after a prolonged contracture and a transient increase in twitch height. Pa-ll at i0 ~g/ml reduced responses to acetylchollne, carbachol and KCI. At 2 ~g/ml, 50% twitch block was observed after 500 minutes and at 5-20 ~g/ml, complete block was observed after 300 minutes. Pa-13 (at 20 ~g/ml) had no effect on acetylcholine, carbachol and KCl responses, but at 30-40 ~g/ml, these responses were reduced. Neuromuscular block was not observed after 300 minutes at 20-40 ~g/ml. On addition of Pa-13, an immediate, brief contracture occurred. Pa-ll and Pa-13 were tested on mouse hemldlaphragm nerve-muscle preparations stimulated directly and indirectly. The onset of block with Pa-ll was similar at different concentrations, with no lag period. Initially, the blocking rates of the indirect and direct responses were similar but eventually the indirect response was blocked at a faster rate. Only the highest concentration tested (i0 ~g/ml) abolished the direct response. Addition of Pa-ll was followed by muscle contracture and a transient increase in twitch height. Pa-13 (10-20 ~g/ml) caused no increase in indirectly evoked twitches. Contracture occurs at the same time as spontaneous twitching. Neuromuscular block was not observed after 300 minutes.