SERRS-based enzymatic probes for the detection of protease activity

Ingram, A. and Byers, L. and Faulds, K. and Moore, B.D. and Graham, D. (2008) SERRS-based enzymatic probes for the detection of protease activity. Journal of the American Chemical Society, 130 (36). pp. 11846-11847. ISSN 1520-5126 (https://doi.org/10.1021/ja803655h)

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Abstract

Measurement of protease activity, for the first time using SERRS as a detection method, is reported herein. Synthetic introduction of phenylalanine to a benzotriazole azo dye allows the SERRS response to be 'switched off' and subsequent exposure to protease restores the SERRS response. The substrates exhibit varying reactivity for a range of proteases and allow for in situ, real-time analysis of protease reactivity. A limit of detection for one protease, Subtilisin carlsberg, was investigated and was established to be 50 ng ml−1.

ORCID iDs

Ingram, A., Byers, L., Faulds, K. ORCID logoORCID: https://orcid.org/0000-0002-5567-7399, Moore, B.D. ORCID logoORCID: https://orcid.org/0000-0002-4943-1632 and Graham, D. ORCID logoORCID: https://orcid.org/0000-0002-6079-2105;
CORE (COnnecting REpositories)