Resonance raman-spectroscopy of hemoglobin in intact-cells: A probe of oxygen-uptake by erythrocytes in rheumatoid arthritis

Hoey, S. and Brown, D.H. and McConnell, A.A. and Smith, W.E. and Marabani, M. and Sturrock, R.D. (1988) Resonance raman-spectroscopy of hemoglobin in intact-cells: A probe of oxygen-uptake by erythrocytes in rheumatoid arthritis. Journal of Inorganic Biochemistry, 34 (3). pp. 189-199. ISSN 0162-0134 (http://dx.doi.org/10.1016/0162-0134(88)85029-3)

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Abstract

Resonance Raman spectra from intact viable erythrocytes can be used to study oxygen uptake in solution. In addition to changes in the oxidation state marker (v4), other bands due to the porphyrin ring (v3) and vinyl modes indicate subtle changes at oxygen pressures close to where the T/R change occurs. A comparison of whole cell and lysate spectra indicates a partial denaturation of hemoglobin on lysis. A simple smear technique is used to measure spectra from rheumatoid and normal blood. Results indicate a faster but less complete uptake of oxygen in cells from patients with rheumatoid arthritis than is the case in normal cell populations.

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