A f-19 and deuterium nmr-study of two novel fluorosterols and their properties in model membranes and high-density-lipoprotein
Reid, David G. and MacLachlan, L.K. and Suckling, K.E. and Gee, A. and Cresswell, S. and Suckling, C.J. (1991) A f-19 and deuterium nmr-study of two novel fluorosterols and their properties in model membranes and high-density-lipoprotein. Chemistry and Physics of Lipids, 58 (1-2). pp. 175-181. ISSN 0009-3084
Full text not available in this repository.Request a copyAbstract
Fluorine-19 nuclear magnetic resonance at 235 MHz has been used to characterize the NMR properties of two novel fluorinated sterols, 6,6-difluorocholestanol and 7,7-difluorocholestanol, and various 3-ester derivatives. F-19-F-19 and F-19-H-1 coupling constants in chloroform are reported. H-2-NMR studies of side-chain perdeuterated dimyristoylphosphatidylcholine (DMPC-d54) prove that the two unesterified fluorosterols affect model membrane dynamical properties in a manner analogous to cholesterol. In aqueous detergent the F-19 chemical shifts of both 6,6- and 7,7-difluorocholestanols are perturbed about 1 ppm upfield by esterification at the 3-position, suggesting that F-19-NMR may be a useful monitor of enzyme catalyzed sterol acylation in vitro. Finally 6,6-difluorocholestanol oleate has been incorporated into human high density lipoprotein (HDL) and its F-19 signals observed in this environment. The appearance of the spectrum, and the results of lipophilic relaxation probe experiments, indicate that the acyl sterol partitions into at least two environments, and exhibits slightly different chemical shift and homonuclear scalar coupling characteristics in each.
-
-
Item type: Article ID code: 16783 Dates: DateEventMay 1991PublishedSubjects: Science > Chemistry Department: Faculty of Science > Pure and Applied Chemistry Depositing user: Strathprints Administrator Date deposited: 19 Mar 2010 15:32 Last modified: 08 Apr 2024 17:19 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/16783