Expression of cre recombinase during transient phage infection permits efficeient marker removal in streptomyces

Khodakaramian, G. and Gust, B. and Lissenden, S. and Moir, L. and Hoskisson, P. and Chater, K.F. and Smith, M.C.M. (2006) Expression of cre recombinase during transient phage infection permits efficeient marker removal in streptomyces. Nucleic Acids Research, 34. E20. ISSN 0305-1048

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Abstract

We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage fC31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor.