Quantifying antibody binding : techniques and therapeutic implications

Tools

Lodge, James and Kajtar, Lewis and Duxbury, Rachel and Hall, David and Burley, Glenn A. and Cordy, Joanna and Yates, James W.T. and Rattray, Zahra (2025) Quantifying antibody binding : techniques and therapeutic implications. mAbs, 17 (1). 2459795. ISSN 1942-0870 (https://doi.org/10.1080/19420862.2025.2459795)

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Abstract

The binding kinetics of an antibody for its target antigen represent key determinants of its biological function and success as a novel biotherapeutic. Defining these interactions and kinetics is critical for understanding the pharmacological and pharmacodynamic profiles of antibodies in therapeutic applications, with line of sight to clinical translation. In this review, we discuss the latest developments in approaches to measure and modulate antibody-antigen interactions, including antibody engineering, novel antibody formats, current, and emerging technologies for measuring antibody-antigen binding interactions, and emerging perspectives within the field. We also explore how emerging computational methods are set to become powerful tools for modeling antibody-binding interactions under physiologically relevant conditions. Finally, we consider the therapeutic implications of modulating target engagement in terms of pharmacodynamics and pharmacokinetics.

ORCID iDs

Lodge, James, Kajtar, Lewis, Duxbury, Rachel, Hall, David, Burley, Glenn A. ORCID logoORCID: https://orcid.org/0000-0002-4896-113X, Cordy, Joanna, Yates, James W.T. and Rattray, Zahra ORCID logoORCID: https://orcid.org/0000-0002-8371-8549;
CORE (COnnecting REpositories)