Obtaining super-resolved images at the mesoscale through super-resolution radial fluctuations

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Brown, Mollie and Foylan, Shannan and Rooney, Liam M. and Gould, Gwyn and McConnell, Gail (2024) Obtaining super-resolved images at the mesoscale through super-resolution radial fluctuations. Journal of Biomedical Optics, 29 (12). 126502. ISSN 1083-3668 (https://doi.org/10.1117/1.JBO.29.12.126502)

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Abstract

Significance: Current super-resolution imaging techniques allow for a greater understanding of cellular structures however they are often complex or only have the ability to image a few cells at once. This small field of view may not represent the behavior across the entire sample, and manual selection of ROIs may introduce bias. It is possible to stitch and tile many small ROI, however this can result in artefacts across an image. Aim: To achieve accurate super-resolved images across a large field of view (4.4 mm x 3.0 mm). Approach: We have applied Super-Resolution Radial Fluctuations processing in conjunction with the Mesolens, which has the unusual combination of a low-magnification and high numerical aperture, to obtain super-resolved images. Results: We demonstrate it is possible to achieve images with a resolution of 446.3 ± 10.9 nm, providing a ~1.6-fold improvement in spatial resolution, over a field of view of 4.4 mm x 3.0 mm, with minimal error, and consistent structural agreement. Conclusions: This work provides a simple method for obtaining accurate super-resolution images over a large FOV, allowing for a simultaneous understanding of both subcellular structures and their large-scale interactions.

ORCID iDs

Brown, Mollie ORCID logoORCID: https://orcid.org/0009-0009-6433-2470, Foylan, Shannan, Rooney, Liam M. ORCID logoORCID: https://orcid.org/0000-0002-2237-501X, Gould, Gwyn ORCID logoORCID: https://orcid.org/0000-0001-6571-2875 and McConnell, Gail ORCID logoORCID: https://orcid.org/0000-0002-7213-0686;
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