Structured illumination microscopy using micro-pixellated light-emitting diodes
Poher, V. and Kennedy, G.T. and Elson, D.S. and French, P.M.W. and Neil, M.A.A. and Zhang, H.Z. and Gu, E. and Gong, Z. and Griffin, C. and Girkin, J.M. and Dawson, M.D. (2006) Structured illumination microscopy using micro-pixellated light-emitting diodes. In: Photon 06, 2006-09-04 - 2006-09-07. (Unpublished) (http://photon06archive.iopconfs.org/Advanced%20ima...)
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Abstract
Structured illumination is a flexible and economical method of obtaining optical sectioning in wide-field microscopy [1]. In this technique the illumination system is modified to project a single-spatial frequency grid pattern onto the sample [2, 3]. The pattern can only be resolved in the focal plane and by recording images for different transverse grid positions (or phases) an image of the in-focus parts of the object can be calculated. Light emitting diodes (LEDs) are becoming increasingly popular for lighting and illumination systems due to their low cost, small dimensions, low coherence, uniform illumination, high efficiency and long lifetime. These properties, together with recent developments in high brightness, ultraviolet operation and microstructured emitter design offer great potential for LEDs as light sources for microscopy. In this paper we demonstrate a novel structured illumination microscope using a blue micro-structured light emitting diode as the illumination source. The system is potentially very compact and has no-moving-parts.
ORCID iDs
Poher, V., Kennedy, G.T., Elson, D.S., French, P.M.W., Neil, M.A.A., Zhang, H.Z., Gu, E. ORCID: https://orcid.org/0000-0002-7607-9902, Gong, Z., Griffin, C., Girkin, J.M. and Dawson, M.D. ORCID: https://orcid.org/0000-0002-6639-2989;-
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Item type: Conference or Workshop Item(Paper) ID code: 9114 Dates: DateEvent2006PublishedSubjects: Science > Physics > Optics. Light Department: Faculty of Science > Physics > Institute of Photonics Depositing user: Strathprints Administrator Date deposited: 13 Nov 2009 11:04 Last modified: 11 Nov 2024 16:15 URI: https://strathprints.strath.ac.uk/id/eprint/9114