Decline in Isotope Dilution Space Ratio Above Age 60 Could Affect Energy Estimates Using the Doubly Labeled Water Method

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Wong, William W and Speakman, John R and Ainslie, Philip N and Anderson, Liam J and Arab, Leonore and Baddou, Issad and Bedu-Addo, Kweku and Blaak, Ellen E and Blanc, Stephane and Bonomi, Alberto G and Bouten, Carlijn V C and Bovet, Pascal and Buchowski, Maciej S and Butte, Nancy F and Camps, Stefan G and Casper, Regina and Close, Graeme L and Colbert, Lisa H and Cooper, Jamie A and Das, Sai K and Davies, Peter S W and Eaton, Simon and Ekelund, Ulf and Hambly, Catherine and El Hamdouchi, Asmaa and Entringer, Sonja and Fudge, Barry W and Gillingham, Melanie and Goris, Annelies H and Gurven, Micheal and Hoos, Marije B and Hu, Sumei and Joosen, Annemiek and Katzmarzyk, Peter T and Kempen, Kitty P and Kimura, Misaka and Kraus, William E and Kushner, Robert F and Larsson, Christel L and Morehen, James C and Morton, James P and Neuhouser, Marian L and Nicklas, Theresa A and Ojiambo, Robert M and Pietilainen, Kirsi H and Pitsiladis, Yannis P and Plasqui, Guy and Reilly, John J and Valenti, Giulio and Wells, Jonathan C K, IAEA DLW Database Consortium (2024) Decline in Isotope Dilution Space Ratio Above Age 60 Could Affect Energy Estimates Using the Doubly Labeled Water Method. Journal of Nutrition, 154 (12). pp. 3824-3831. ISSN 0022-3166 (https://doi.org/10.1016/j.tjnut.2024.10.016)

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Abstract

Background: Doubly labeled water is gold standard for measuring total energy expenditure (TEE). Measurements using the method are sensitive to the isotope dilution space ratio (DSR). Accuracy and precision of the method might be improved if we could identify factors influencing DSR. Objectives: We evaluated the potential associations of age, sex, ethnicity, anthropometry, body composition, turnover rates of the isotopes, and geographical elevation with DSR. Methods: We used univariate regression analysis to explore the relationships between the continuous variables and analysis of variance to test the relationships between the categorical variables with DSR. Subsequently, we used general linear model (GLM) and 1-way analysis of variance to evaluate the simultaneous associations of age, sex, ethnicity, fat-free mass (FFM) and fat mass (FM) on DSR. Results: From 5678 measurements complied from studies around the world with diverse ethnicity and living at various elevations, the mean DSR was 1.0364 ± 0.0141. No meaningful physiologic effect of any of the continuous and categorical variable on DSR was detected. General linear model analysis revealed no effect of FFM and FM (P > 0.33) on DSR, but DSR decreased with age (P < 0.001) among those aged 60 y and older regardless of sex. Among the Whites who were younger than 60 y, DSR was not related to FFM and FM (P = 0.73) but was affected by both age and sex (P < 0.001). Conclusions: Previous estimates of age-related decline in TEE may have overestimated TEE at age 90 y. Validation studies on older participants are required to confirm this finding.

ORCID iDs

Wong, William W, Speakman, John R, Ainslie, Philip N, Anderson, Liam J, Arab, Leonore, Baddou, Issad, Bedu-Addo, Kweku, Blaak, Ellen E, Blanc, Stephane, Bonomi, Alberto G, Bouten, Carlijn V C, Bovet, Pascal, Buchowski, Maciej S, Butte, Nancy F, Camps, Stefan G, Casper, Regina, Close, Graeme L, Colbert, Lisa H, Cooper, Jamie A, Das, Sai K, Davies, Peter S W, Eaton, Simon, Ekelund, Ulf, Hambly, Catherine, El Hamdouchi, Asmaa, Entringer, Sonja, Fudge, Barry W, Gillingham, Melanie, Goris, Annelies H, Gurven, Micheal, Hoos, Marije B, Hu, Sumei, Joosen, Annemiek, Katzmarzyk, Peter T, Kempen, Kitty P, Kimura, Misaka, Kraus, William E, Kushner, Robert F, Larsson, Christel L, Morehen, James C, Morton, James P, Neuhouser, Marian L, Nicklas, Theresa A, Ojiambo, Robert M, Pietilainen, Kirsi H, Pitsiladis, Yannis P, Plasqui, Guy, Reilly, John J ORCID logoORCID: https://orcid.org/0000-0001-6165-5471, Valenti, Giulio and Wells, Jonathan C K;
CORE (COnnecting REpositories)