A practical implementation of adaptive optics for aberration compensation in optical sectioning microscopy

Wright, A. and Poland, S.P. and Vijverberg, J. and Girkin, J.M.; Dainty, C., ed. (2007) A practical implementation of adaptive optics for aberration compensation in optical sectioning microscopy. In: Adaptive Optics For Industry And Medicine: Proceedings of the Sixth International Workshop. Imperial College Press, London, United Kingdom, pp. 382-387. ISBN 9781848161108 (http://dx.doi.org/10.1142/9781848161115_0063)

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Abstract

We report on a practical method for the implementation of Adaptive Optics in optical sectioning microscopy to remove system and sample induced aberrations. Correcting for induced aberrations on a pixel-by-pixel basis would take in excess of 4 minutes and greatly increase the risk of sample damage due to photo-bleaching and photo-toxicity; this is clearly an impractical approach. We show that a single aberration correction per optical slice is adequate to significantly improve the image quality across the whole field of view. We present results illustrating the success of this method for a sample scanning and beam scanning system using confocal and multiphoton microscopy.

  • Item type:Book Section
    ID code:9073
    Dates:
    Date
    Event
    2007
    Published
    Keywords:adaptive optics, optical sectioning microscopy, aberrations, photo-bleaching, photo-toxicity, beam scanning system, confocal, multiphoton, Optics. Light
    Subjects:Science > Physics > Optics. Light
    Department:Faculty of Science > Physics > Institute of Photonics
    Depositing user: Strathprints Administrator
    Date deposited:02 Nov 2009 12:25
    Last modified:18 Jan 2023 12:10
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/9073
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