Microbial interactions in the mosquito gut determine Serratia colonization and blood-feeding propensity

Kozlova, Elena V. and Hegde, Shivanand and Roundy, Christopher M. and Golovko, George and Saldaña, Miguel A. and Hart, Charles E. and Anderson, Enyia R. and Hornett, Emily A. and Khanipov, Kamil and Popov, Vsevolod L. and Pimenova, Maria and Zhou, Yiyang and Fovanov, Yuriy and Weaver, Scott C. and Routh, Andrew L. and Heinz, Eva and Hughes, Grant L. (2021) Microbial interactions in the mosquito gut determine Serratia colonization and blood-feeding propensity. ISME Journal, 15 (1). pp. 93-108. ISSN 1751-7362 (https://doi.org/10.1038/s41396-020-00763-3)

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Abstract

How microbe–microbe interactions dictate microbial complexity in the mosquito gut is unclear. Previously we found that, Serratia, a gut symbiont that alters vector competence and is being considered for vector control, poorly colonized Aedes aegypti yet was abundant in Culex quinquefasciatus reared under identical conditions. To investigate the incompatibility between Serratia and Ae. aegypti, we characterized two distinct strains of Serratia marcescens from Cx. quinquefasciatus and examined their ability to infect Ae. aegypti. Both Serratia strains poorly infected Ae. aegypti, but when microbiome homeostasis was disrupted, the prevalence and titers of Serratia were similar to the infection in its native host. Examination of multiple genetically diverse Ae. aegypti lines found microbial interference to S. marcescens was commonplace, however, one line of Ae. aegypti was susceptible to infection. Microbiome analysis of resistant and susceptible lines indicated an inverse correlation between Enterobacteriaceae bacteria and Serratia, and experimental co-infections in a gnotobiotic system recapitulated the interference phenotype. Furthermore, we observed an effect on host behavior; Serratia exposure to Ae. aegypti disrupted their feeding behavior, and this phenotype was also reliant on interactions with their native microbiota. Our work highlights the complexity of host–microbe interactions and provides evidence that microbial interactions influence mosquito behavior.

ORCID iDs

Kozlova, Elena V., Hegde, Shivanand, Roundy, Christopher M., Golovko, George, Saldaña, Miguel A., Hart, Charles E., Anderson, Enyia R., Hornett, Emily A., Khanipov, Kamil, Popov, Vsevolod L., Pimenova, Maria, Zhou, Yiyang, Fovanov, Yuriy, Weaver, Scott C., Routh, Andrew L., Heinz, Eva ORCID logoORCID: https://orcid.org/0000-0003-4413-3756 and Hughes, Grant L.;
  • Item type:Article
    ID code:90696
    Dates:
    Date
    Event
    January 2021
    Published
    7 September 2020
    Published Online
    25 August 2020
    Accepted
    Subjects:Science > Microbiology
    Department:UNSPECIFIED
    Depositing user: Pure Administrator
    Date deposited:24 Sep 2024 15:25
    Last modified:11 Nov 2024 14:27
    Related URLs:
    URI:https://strathprints.strath.ac.uk/id/eprint/90696
CORE (COnnecting REpositories)