The antibacterial efficacy of far-UVC light : a combined-method study exploring the effects of experimental and bacterial variables on dose-response

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Griffin, David T. and Gourlay, Terence and Maclean, Michelle (2024) The antibacterial efficacy of far-UVC light : a combined-method study exploring the effects of experimental and bacterial variables on dose-response. Pathogens, 13 (8). 698. ISSN 2076-0817 (https://doi.org/10.3390/pathogens13080698)

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Abstract

Far-ultraviolet C light, with a wavelength of 200–230 nm, has demonstrated broad-spectrum germicidal efficacy. However, due to increased interest in its use as an alternative antimicrobial, further knowledge about its fundamental bactericidal efficacy is required. This study had two objectives. Firstly, it investigated experimentally the Far-UVC dose–response of common bacteria suspended at various cell densities in transparent buffer, ensuring no influence from photosensitive suspending media. Increasing doses of Far-UVC were delivered to Enterococcus faecium, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus in PBS at 101, 102, 103, 105 and 107 CFU·mL−1, with surviving colony-forming units enumerated (n ≥ 3). Secondly, through a systematised literature review, this work sought to explore the impact of genus/species, Gram type, cell form, cell density and irradiance on dose–response. The screening of 483 publications was performed with 25 included in the study. Data for 30 species were collated, analysed and compared with the experimental results. Overall, Gram-positive species showed greater resilience to Far-UVC than Gram-negative; some inter-species and inter-genera differences in resilience were identified; endospores were more resilient than vegetative cells; the results suggested that inactivation efficiency may decrease as cell density increases; and no significant correlation was identified between irradiance and bactericidal dose effect. In conclusion, this study has shown Far-UVC light to be an effective decontamination tool against a vast range of bacterial vegetative cells and endospores.

ORCID iDs

Griffin, David T., Gourlay, Terence and Maclean, Michelle ORCID logoORCID: https://orcid.org/0000-0001-5750-0397;
CORE (COnnecting REpositories)