Using 3D printed optical elements for multifocal image scanning microscopy

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Christopher, Jay and Donnachie, Mark and Rooney, Liam and McConnell, Gail and Uttamchandani, Deepak and Bauer, Ralf (2024) Using 3D printed optical elements for multifocal image scanning microscopy. Proceedings of SPIE, 12827. 1282702. ISSN 0277-786X (https://doi.org/10.1117/12.3002507)

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Abstract

We present a small footprint and affordable implementation of a multifocal image scanning microscope (ISM), utilizing both a microelectromechanical system (MEMS) micromirror for flexible optical excitation control, and multifocal patterned illumination using a custom 3D printed optical quality lenslet array. We highlight the individual element performance and demonstrate its use in fluorescence imaging to allow comparison of the affordable and customizable approach with its commercial counterpart.

ORCID iDs

Christopher, Jay ORCID logoORCID: https://orcid.org/0009-0009-0707-8947, Donnachie, Mark ORCID logoORCID: https://orcid.org/0000-0001-5391-236X, Rooney, Liam ORCID logoORCID: https://orcid.org/0000-0002-2237-501X, McConnell, Gail ORCID logoORCID: https://orcid.org/0000-0002-7213-0686, Uttamchandani, Deepak ORCID logoORCID: https://orcid.org/0000-0002-2362-4874 and Bauer, Ralf ORCID logoORCID: https://orcid.org/0000-0001-7927-9435;
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