Using 3D printed optical elements for multifocal image scanning microscopy

Christopher, Jay and Donnachie, Mark and Rooney, Liam and McConnell, Gail and Uttamchandani, Deepak and Bauer, Ralf (2024) Using 3D printed optical elements for multifocal image scanning microscopy. Proceedings of SPIE, 12827. 1282702. ISSN 0277-786X (https://doi.org/10.1117/12.3002507)

[thumbnail of Christopher-etal-SPIE-2024-Using-3D-printed-optical-elements-for-multifocal-image-scanning-microscopy]
Preview
 Text. Filename: Christopher-etal-SPIE-2024-Using-3D-printed-optical-elements-for-multifocal-image-scanning-microscopy.pdf
Accepted Author Manuscript
License: Creative Commons Attribution 4.0 logo
Download (1MB)| Preview

Abstract

We present a small footprint and affordable implementation of a multifocal image scanning microscope (ISM), utilizing both a microelectromechanical system (MEMS) micromirror for flexible optical excitation control, and multifocal patterned illumination using a custom 3D printed optical quality lenslet array. We highlight the individual element performance and demonstrate its use in fluorescence imaging to allow comparison of the affordable and customizable approach with its commercial counterpart.

Persistent Identifier

https://doi.org/10.17868/strath.00089844
CORE (COnnecting REpositories)