Sequence and origin of the Streptomyces intergenetic-conjugation helper plasmid pUZ8002
Larcombe, Daniel E. and Braes, Robyn E. and Croxford, James T. and Wilson, James W. and Figurski, David H. and Hoskisson, Paul A. (2024) Sequence and origin of the Streptomyces intergenetic-conjugation helper plasmid pUZ8002. Access Microbiology, 6 (6). 000808. ISSN 2516-8290 (https://doi.org/10.1099/acmi.0.000808.v3)
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Abstract
Conjugation of plasmids from Escherichia coli is essential for the genetic manipulation of Streptomyces spp. To facilitate intergeneric conjugation from E. coli to Streptomyces the conjugative machinery required for genetic transfer is usually provided by the non-transferable helper plasmid, pUZ8002. Here we present the complete nucleotide sequence of pUZ8002, describe the previously undocumented creation process, and provide details of the sequence relative to the parental pUZ8 plasmid and another previously published pUZ8002 sequence.
ORCID iDs
Larcombe, Daniel E., Braes, Robyn E., Croxford, James T., Wilson, James W., Figurski, David H. and Hoskisson, Paul A. ORCID: https://orcid.org/0000-0003-4332-1640;-
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Item type: Article ID code: 89271 Dates: DateEvent5 June 2024Published13 May 2024AcceptedSubjects: Science > Microbiology Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 17 May 2024 13:04 Last modified: 12 Dec 2024 15:28 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/89271