Polarised light modular microscopy (Pol-ModMicro) for identifying hemozoin crystals in Plasmodium
Eadie, Fraser and Gibbins, Matthew P and Gibson, Graham and Marti, Matthias and Kallepalli, Akhil (2023) Polarised light modular microscopy (Pol-ModMicro) for identifying hemozoin crystals in Plasmodium. Other. arXiv.
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Abstract
Plasmodium spp. are the protozoan parasites responsible for malaria. Plasmodium spp. synthesise a biocrystal, hemozoin, which can be observed under cross-polarised light. These birefringent crystals can be seen due to different refractive indices of the hemozoin crystal and red blood cells. Here, we present a polarised light modular microscopy (Pol-ModMicro) solution, complete with illumination sources and a robust imaging system to capture birefringence and identify the parasites. We achieve this by combining ModLight light sources with bespoke components designed for the OpenFlexure microscope to image blood smears. Further, a simple and robust algorithm capable of enhancing birefringence is presented. This solution provides image quality that is comparable to a substantially more expensive proprietary microscope.
ORCID iDs
Eadie, Fraser ORCID: https://orcid.org/0009-0001-4117-4125, Gibbins, Matthew P, Gibson, Graham, Marti, Matthias and Kallepalli, Akhil ORCID: https://orcid.org/0000-0001-8115-9379;-
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Item type: Monograph(Other) ID code: 89002 Dates: DateEvent26 April 2023PublishedSubjects: Medicine > Biomedical engineering. Electronics. Instrumentation Department: Faculty of Engineering > Biomedical Engineering Depositing user: Pure Administrator Date deposited: 26 Apr 2024 14:55 Last modified: 12 Dec 2024 01:59 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/89002