Self-decorating cells via surface-initiated enzymatic controlled radical polymerization

Belluati, Andrea and Happel, Dominic and Erbe, Malte and Kirchner, Nicole and Szelwicka, Anna and Bloch, Adrian and Berner, Valeria and Christmann, Andreas and Hertel, Brigitte and Pardehkhorram, Raheleh and Reyhani, Amin and Kolmar, Harald and Bruns, Nico (2023) Self-decorating cells via surface-initiated enzymatic controlled radical polymerization. Nanoscale, 15 (48). pp. 19486-19492. ISSN 2040-3372 (https://doi.org/10.1039/D3NR04008A)

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Abstract

Through the innovative use of surface-displayed horseradish peroxidase, this work explores the enzymatic catalysis of both bioRAFT polymerization and bioATRP to prompt polymer synthesis on the surface of Saccharomyces cerevisiae cells, with bioATRP outperforming bioRAFT polymerization. The resulting surface modification of living yeast cells with synthetic polymers allows for a significant change in yeast phenotype, including growth profile, aggregation characteristics, and conjugation of non-native enzymes to the clickable polymers on the cell surface, opening new avenues in bioorthogonal cell-surface engineering.

  • Item type:Article
    ID code:87597
    Dates:
    Date
    Event
    28 December 2023
    Published
    1 December 2023
    Published Online
    30 November 2023
    Accepted
    Subjects:Science > Chemistry
    Department:Faculty of Science > Pure and Applied Chemistry
    Depositing user: Pure Administrator
    Date deposited:12 Dec 2023 14:18
    Last modified:24 Apr 2024 00:30
    URI:https://strathprints.strath.ac.uk/id/eprint/87597
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