GLUT4 dispersal at the plasma membrane of adipocytes : a super-resolved journey
Geiser, Angéline and Foylan, Shannan and Tinning, Peter W. and Bryant, Nia J. and Gould, Gwyn W. (2023) GLUT4 dispersal at the plasma membrane of adipocytes : a super-resolved journey. Bioscience Reports, 43 (10). BSR20230946. ISSN 1573-4935 (https://doi.org/10.1042/BSR20230946)
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Abstract
In adipose tissue, insulin stimulates glucose uptake by mediating the translocation of GLUT4 from intracellular vesicles to the plasma membrane. In 2010, insulin was revealed to also have a fundamental impact on the spatial distribution of GLUT4 within the plasma membrane, with the existence of two GLUT4 populations at the plasma membrane being defined: 1) as stationary clusters and 2) as diffusible monomers. In this model, in the absence of insulin, plasma membrane-fused GLUT4 are found to behave as clusters. These clusters are thought to arise from an exocytic event that retains GLUT4 at the fusion site; this has been proposed to function as an intermediate hub between GLUT4 exocytosis and re-internalisation. By contrast, insulin stimulation induces the dispersal of GLUT4 clusters into monomers and favors a distinct type of GLUT4-vesicle fusion event, known as fusion-with-release exocytosis. Here, we review how super-resolution microscopy approaches have allowed investigation of the characteristics of plasma membrane-fused GLUT4 and further discuss regulatory step(s) involved in the GLUT4 dispersal machinery, introducing the scaffold protein EFR3 which facilitates localisation of phosphatidylinositol 4-kinase type IIIα (PI4KIIIα) to the cell surface. We consider how dispersal may be linked to the control of transporter activity, consider whether macro-organisation may be a widely used phenomenon to control proteins within the plasma membrane, and speculate on the origin of different forms of GLUT4-vesicle exocytosis.
ORCID iDs
Geiser, Angéline ORCID: https://orcid.org/0000-0002-7108-2440, Foylan, Shannan, Tinning, Peter W. ORCID: https://orcid.org/0000-0002-3270-4120, Bryant, Nia J. and Gould, Gwyn W. ORCID: https://orcid.org/0000-0001-6571-2875;-
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Item type: Article ID code: 86985 Dates: DateEvent20 October 2023Published4 October 2023Accepted7 September 2023SubmittedSubjects: Science > Natural history > Biology
Medicine > Medicine (General)Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 18 Oct 2023 10:52 Last modified: 02 Oct 2024 00:41 URI: https://strathprints.strath.ac.uk/id/eprint/86985